In vitro and in vivo susceptibility of the honeybee bacterial pathogen Paenibacillus larvae subsp. larvae to the antibiotic tylosin

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In vitro and in vivo susceptibility of the honeybee bacterial pathogen Paenibacillus larvae subsp. larvae to the antibiotic tylosin.

The minimal inhibitory concentrations (MICs) of tylosin were determined to 67 strains of Paenibacillus larvae subsp. larvae, the causal agent of American Foulbrood (AFB) disease, from different geographical origins. MIC values obtained ranged from 0.0078 to 0.5 microg/ml. These very low values imply that no resistance to tylosin was found in any isolate of the Foulbrood pathogen. The measuremen...

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Secreted and immunogenic proteins produced by the honeybee bacterial pathogen, Paenibacillus larvae.

American Foulbrood is a severe disease affecting larvae of honeybee Apis mellifera, causing significant decrease in the honeybee population, beekeeping industries and agricultural production. In spite of its importance, little is known about the virulence factors secreted by Paenibacillus larvae during larval infection. The aim of the present work was to perform a first approach to the identifi...

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Transcriptional Response of Honey Bee Larvae Infected with the Bacterial Pathogen Paenibacillus larvae

American foulbrood disease of honey bees is caused by the bacterium Paenibacillus larvae. Infection occurs per os in larvae and systemic infection requires a breaching of the host peritrophic matrix and midgut epithelium. Genetic variation exists for both bacterial virulence and host resistance, and a general immunity is achieved by larvae as they age, the basis of which has not been identified...

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Detection of Paenibacillus larvae larvae spores in honey and diseased larvae Samples by culture and PCR

American foulbrood (AFB) is the most serious brood disease of the honey bee. Traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. The aim of this study is the detection of Paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and PCR. Therefore 54 samp...

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Transcriptional immune responses by honey bee larvae during invasion by the bacterial pathogen, Paenibacillus larvae.

Honey bee larvae are highly susceptible to the bacterial pathogen Paenibacillus larvae only during the first instar of larval development. Transcript levels were measured for genes encoding two antimicrobial peptides, abaecin and defensin, as well as for two candidates in the immune response cascade (PGRP-LD and masquerade) in control larvae and larvae exposed to the pathogen. Transcripts for a...

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ژورنال

عنوان ژورنال: Veterinary Microbiology

سال: 2005

ISSN: 0378-1135

DOI: 10.1016/j.vetmic.2005.03.008